Novel Mediator of T Cell Tolerance Granulocyte Colony - Stimulating Factor : A and Giuseppe Leone

of April 12 , 2017 . This information is current as Novel Mediator of T Cell Tolerance Granulocyte Colony - Stimulating Factor : A and Giuseppe

Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)

Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...

Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...

Effect of Enteral Administration of Granulocyte-Colony Stimulating Factor (G-CSF) on Feeding Tolerance in Very Low Birth Weight and Extremely Low Birth Weight Neonates; a Historical-Controlled Clinical Trial

Background: The current study aimed to investigate the effect of enteral Granulocyte-Colony Stimulating G-CSF(Factor) on feeding tolerance in very low birth weight (VLBW) and extremely low birth weight (ELBW) neonates.Methods: This historical-controlled clinical trial was conducted on VLBW and ELBW neonates admitted to MahdiehHospital, affiliated to Shahid Beheshti University ...

Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Macrophage Colony-Stimulating Factor (hGM-CSF) in Escherichia coli and Its Recognition by Reciprocal Antibodies

Fusion of two genes at DNA level produces a single protein, known as a chimeric protein. Immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. Bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1) was fused to human granulocyte macrophage ...